Reporter

Part:BBa_K4799608

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtPCR2_GUS_T-Nos

pAtPCR2 is a promoter. It is added before the reporter gene β-Glucuronidase (gus) and NOS terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 22
    Illegal NheI site found at 3852
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 22
    Illegal BglII site found at 1834
    Illegal BamHI site found at 1817
    Illegal XhoI site found at 477
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
    Illegal NgoMIV site found at 2267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2067
    Illegal BsaI.rc site found at 2682
    Illegal SapI.rc site found at 2853


[edit]
Categories
Parameters
None